Nick Bosa played every snap despite dealing with a rib injury, showcasing his determination and impact on the field. Alongside Bosa, other defensive linemen like Maliek Collins, Hargrave, Leonard Floyd, and more contributed to the team's defensive efforts.

Debuting in his first game from the practice squad, Sam Okuayinonu made a notable impact by recording his first full sack of his career. His performance helped solidify the defensive lineup, with key players like Jordan Elliott also stepping up in their roles. The 49ers' defensive strategies, such as utilizing a five-lineman front with effective run stuffers like Elliott, showcased their adaptability throughout the game.

While the defensive line showcased strength and resilience, the cornerback positions faced challenges. Charvarius Ward struggled due to knee and hamstring injuries, allowing Renardo Green to step in during certain snaps. Additionally, Isaac Yiadom faced targeted plays by opponents, highlighting areas for improvement in the secondary defense. Despite these challenges, the team worked collectively to mitigate defensive gaps and adapt to evolving game situations.